Coding

Part:BBa_K2587010:Design

Designed by: Susanne Vollmer   Group: iGEM18_Duesseldorf   (2018-09-18)


trzC_opt


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
    Illegal NotI site found at 2
    Illegal NotI site found at 1294
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25
    Illegal BsaI.rc site found at 1280


Design Notes

Codon optimized for S. cerevisiae. Added overhangs for golden gate cloning with CIDAR MoClo Toolbox (Iverson et al., 2015)


Source

Synthesized codon optimized for S. cerevisiae from IDT.

References